BUB3 antibody (AA 275-325)

Catalog No. ABIN7449397

This Rabbit Polyclonal antibody specifically detects BUB3 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for BUB3 antibody (AA 275-325) (ABIN7449397)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BUB3 Antibody

Binding Specificity: AA 275-325

Purpose: Rabbit anti-Bub3 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 275 and 325

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

WB: 1:5,000 - 1:15,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: Bub3

Background: Background: Bub3 is an adaptor protein and component of the spindle checkpoint machinery. Bub3 and other spindle checkpoint proteins ensure that segregation does not proceed until all replicated chromatids are associated with a functional bipolar spindle. Bub3 forms a constitutive complex with Bub1. Bub3 has been demonstrated to also function as a transcriptional repressor in association with Cdc20 and histone deacetylases.

Gene ID: 9184

NCBI Accession: NP_004716

UniProt: O43684

Pathways: Maintenance of Protein Location