Recombinant GAPDH antibody (AA 225-275)

Catalog No. ABIN7449428

The Rabbit Monoclonal anti-GAPDH antibody has been validated for WB, IHC and ICC. It is suitable to detect GAPDH in samples from Human and Mouse.

Quick Overview for Recombinant GAPDH antibody (AA 225-275) (ABIN7449428)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: BLR103H

Product Details anti-GAPDH Antibody

Binding Specificity: AA 225-275

Purpose: Rabbit anti-GAPDH Recombinant Monoclonal Antibody [BLR103H]

Immunogen: between AA 225 and 275

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 01:01,00

Restrictions: For Research Use only

Handling

Concentration: 30 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Background: In the process of carbohydrate metabolism, GAPDH (glyceraldehydes-3-phosphate dehydrogenase) catalyzes the oxidative phosphorylation of glyceraldehydes-3-phosphate to 1,3-diphosphoglycerate in the presence of nicotinamide adenine dinucleotide (NAD) and inorganic phosphate. In addition to its role in the oxidation of glucose, GAPDH has been shown to be involved in other cellular processes such as vesicle transport, DNA repair, DNA replication, cytoskeletal modulation, and tRNA export.

Gene ID: 2597

NCBI Accession: NP_002037

UniProt: P04406