Histone 3 antibody (pSer10)
Quick Overview for Histone 3 antibody (pSer10) (ABIN7449558)
Target
See all Histone 3 (H3) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pSer10
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Purpose
- Rabbit anti-Phospho Histone H3 (S10) Antibody, Affinity Purified
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Predicted Reactivity
- Rat,D. melanogaster,Zebrafish,X. laevis,X. tropicalis,Chicken,Bovine,Rabbit,Pig,Orangutan
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Purification
- Affinity Purified
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Immunogen
- surrounding serine 10
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Isotype
- IgG
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Application Notes
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ICC-IF: 1:2,000 - 1:10,000. Formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely effect staining.
IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
WB: 1:5,000 - 1:10,000
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Restrictions
- For Research Use only
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Concentration
- 200 μg/mL
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Buffer
- Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Expiry Date
- 12 months
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- Histone 3 (H3) (Histone H3 (H3))
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Alternative Name
- Histone H3
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Background
- Background: Histone H3 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.
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Gene ID
- 8350
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NCBI Accession
- NP_003520
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UniProt
- P68431
Target
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