MTOR antibody (AA 1350-1400)

Catalog No. ABIN7449772

This Rabbit Polyclonal antibody specifically detects MTOR in WB and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for MTOR antibody (AA 1350-1400) (ABIN7449772)

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTOR antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-MTOR Antibody

Binding Specificity: AA 1350-1400

Purpose: Rabbit anti-mTOR Antibody, Affinity Purified

Predicted Reactivity: Rat

Purification: Affinity Purified

Immunogen: between AA 1350 and 1400

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Alternative Name: mTOR

Background: Background: Studies of the mammalian target of rapamycin (mTOR) and its homologs demonstrate a role in integrating signals from growth factors, nutrients, stress, and cellular energy levels to control cell growth, translation initiation, ribosome biogenesis, and transcription factor localization. mTOR is the direct target of the cell cycle arresting activity of rapamycin. mTOR interacts with Raptor or Rictor to form the mTORC1 and mTORC2 complexes respectively. The mTORC1 complex also includes mLst8/GbetaL and functions to phosphorylate S6K and 4EBP1. The mTORC2 complex also includes mLst8/GbetaL, mSIN1 and protor-1 and functions to phosphorylate Akt.

Gene ID: 2475

NCBI Accession: NP_004949

UniProt: P42345

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect