MTHFD1 antibody (AA 150-200)

Catalog No. ABIN7450465

This Rabbit Polyclonal antibody specifically detects MTHFD1 in WB and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for MTHFD1 antibody (AA 150-200) (ABIN7450465)

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTHFD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-MTHFD1 Antibody

Binding Specificity: AA 150-200

Purpose: Rabbit anti-MTHFD1 Antibody, Affinity Purified

Predicted Reactivity: Rat,Orangutan

Purification: Affinity Purified

Immunogen: Between AA 150 and 200

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MTHFD1

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Alternative Name: MTHFD1

Background: Background: Methylenetetrahydrofolate dehydrogenase (MTHFD1) possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain [taken from NCBI Entrez Gene (Gene ID: 4522)].

Gene ID: 4522

UniProt: P11586

Pathways: Methionine Biosynthetic Process