GLI2 antibody (AA 950-1000)

Catalog No. ABIN7451134

This Rabbit Polyclonal antibody specifically detects GLI2 in IP. It exhibits reactivity toward Human.

Quick Overview for GLI2 antibody (AA 950-1000) (ABIN7451134)

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI2 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-GLI2 Antibody

Binding Specificity: AA 950-1000

Purpose: Rabbit anti-GLI2 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 950 and 1000

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7451134 has not performed satisfactorily when used for WB of GLI2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of GLI2.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Alternative Name: GLI2

Background: Background: GLI2 functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992). GLI2 may also function as transcriptional repressor (By similarity). It requires STK36 for full transcriptional activator activity. GLI2 is required for normal embryonic development (PubMed:15994174, PubMed:20685856) [taken from NCBI Entrez Gene (Gene ID: 2736)].

Gene ID: 2736

UniProt: P10070

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis