PRKDC antibody (C-Term)

Catalog No. ABIN7451558

The Rabbit Polyclonal anti-PRKDC antibody is suitable to detect PRKDC in samples from Human. It has been validated for WB and IP.

Quick Overview for PRKDC antibody (C-Term) (ABIN7451558)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PRKDC Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-DNA-PKcs Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 4075 and C-term

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate. Ku-80 co-immunoprecipitates with DNA-PKcs when using BL2406.

WB: 1:1,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: DNA-PKcs

Background: Background: DNA-PKcs is the catalytic subunit of a DNA-dependent serine-threonine protein kinase (DNA-PK). DNA-PK is a heterotrimer comprised of DNA-PKcs and the Ku709/Ku86 dimer. DNA-PK is involved in DNA damage repair and V(D)J recombination. DNA-PK phosphorylates Artemis, a protein required for nonhomologous end-joining and is necessary for the loading of Artemis on damaged DNA.

Gene ID: 5591

NCBI Accession: NP_008835

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response