SPAG5 antibody (AA 875-925)

Catalog No. ABIN7451646

Product Details anti-SPAG5 Antibody

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Binding Specificity: AA 875-925

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Purpose: Rabbit anti-Astrin/MAP126 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 875 and 925

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SPAG5

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Alternative Name: Astrin/MAP126 (SPAG5 Products)

Synonyms: SPAG5 antibody, DEEPEST antibody, MAP126 antibody, hMAP126 antibody, AI874642 antibody, D11Bhm180e antibody, Deepest antibody, Mastrin antibody, S17 antibody, sperm associated antigen 5 antibody, sperm-associated antigen 5 antibody, SPAG5 antibody, LOC739263 antibody, Spag5 antibody

Background: Background: Astrin/MAP126 has been identified as a mitotic spindle-associated protein essential for centrosome integrity and the progression through mitosis. Astrin/MAP126 interacts with Ninein and Aurora-A at the centrosome. Ninein, but not Aurora-A, appears to be required for proper localization of Astrin/MAP126 to the centrosome during S and G2 phase. A pool of Astrin/MAP126 also localizes to the outer kinetochore where it plays an essential role in chromatid adhesion and chromosome congression and segregation. The localization of Astrin/MAP126 to the spindle pole and kinetochore has been shown to be dependent on its phosphorylation by glycogen synthase kinase 3beta (GSK3beta).

Gene ID: 10615

NCBI Accession: NP_006452

UniProt: Q96R06

Pathways: M Phase