DFFA antibody (C-Term)

Catalog No. ABIN7451735

The Rabbit Polyclonal anti-DFFA antibody is suitable to detect DFFA in samples from Human. It has been validated for WB and IP.

Quick Overview for DFFA antibody (C-Term) (ABIN7451735)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-DFFA Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-DFF35/DFFA Antibody, Affinity Purified

Predicted Reactivity: Monkey

Purification: Affinity Purified

Immunogen: C-terminus

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DFF35/DFFA

Background: Background: DFF (DNA fragmentation factor) is the principle endonuclease responsible for DNA cleavage during apoptosis and exists as a heterodimer formed between the inhibitory subunit, DFF45, and the DNA nuclease, DFF40. DFF35/DFFA is a 35 kDa splice variant of the inhibitory subunit, DFF45. Similar to DFF45, DFF35 has been shown to heterodimerize with DFF40 and also negatively regulate its nuclease activity. In addition to regulating DFF40 activity, DFF35 has also been found to assist in the synthesis and folding of the DFF40 endonuclease.

Gene ID: 1676

NCBI Accession: NP_998731

UniProt: O00273

Pathways: Apoptosis, Caspase Cascade in Apoptosis