TOPBP1 antibody (C-Term)

Catalog No. ABIN7452107

The Rabbit Polyclonal anti-TOPBP1 antibody has been validated for WB and IP. It is suitable to detect TOPBP1 in samples from Human and Mouse.

Quick Overview for TOPBP1 antibody (C-Term) (ABIN7452107)

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOPBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-TOPBP1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-TopBP1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1400 and C-term

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TOPBP1

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Alternative Name: TopBP1

Background: Background: Topoisomerases regulate topological states of DNA. DNA topoisomerase II-binding protein 1 (TopBP1) is a protein that interacts with the C-terminal region of topoisomerase II beta (Topo II beta). TopBP1 function has been found to be important to various aspects of cellular growth control such as DNA replication, the DNA damage checkpoint, and cell survival. TopBP1 interacts with several proteins through its BRCA1 C-terminal (BRCT) motifs. TopBP1 interacts and inhibits E2F1 transcriptional activity through an Rb-independent mechanism that involves recruitment of the SWI/SNF chromatin-remodeling complex proteins Brg1 and Brm.

Gene ID: 11073

NCBI Accession: NP_008958

UniProt: Q92547

Pathways: Chromatin Binding