ZAP70 antibody (pTyr493)

Catalog No. ABIN7452235

This Rabbit Polyclonal antibody specifically detects ZAP70 in IP. It exhibits reactivity toward Human.

Quick Overview for ZAP70 antibody (pTyr493) (ABIN7452235)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-ZAP70 Antibody

Binding Specificity: pTyr493

Purpose: Rabbit anti-Phospho ZAP70 (Y493) Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: surrounding tyrosine 493

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7452235 has not performed satisfactorily when used for WB of phospho ZAP70 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ZAP70.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: Background: ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.

Gene ID: 7535

NCBI Accession: NP_001070

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway