GTF2F1 antibody (AA 350-400)

Catalog No. ABIN7452243

The Rabbit Polyclonal anti-GTF2F1 antibody (ABIN7452243) specifically detects GTF2F1 in IP. The antibody is reactive with Human samples.

Quick Overview for GTF2F1 antibody (AA 350-400) (ABIN7452243)

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2F1 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-GTF2F1 Antibody

Binding Specificity: AA 350-400

Purpose: Rabbit anti-RAP74 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 350 and 400

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7452243 has not performed satisfactorily when used for WB of RAP74 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RAP74.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for GTF2F1

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Alternative Name: RAP74

Background: Background: The general transcription factor, TFIIF, is composed of a heterodimer of a 74 kDa alpha subunit and a 30 kDa beta subunit. The alpha subunit of TFIIF is also called RAP74. In collaboration with TFIIB, TFIIF helps recruit RNA polymerase II to the pre-initiation complex (PIC).

Gene ID: 2962

NCBI Accession: NP_002087

UniProt: P35269