CNOT1 antibody (AA 1400-1450)

Catalog No. ABIN7452794

The Rabbit Polyclonal anti-CNOT1 antibody is suitable to detect CNOT1 in samples from Human and Mouse. It has been validated for WB and IP.

Quick Overview for CNOT1 antibody (AA 1400-1450) (ABIN7452794)

Target: CNOT1 (CCR4-NOT Transcription Complex, Subunit 1 (CNOT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CNOT1 Antibody

Binding Specificity: AA 1400-1450

Purpose: Rabbit anti-CNOT1 Antibody, Affinity Purified

Predicted Reactivity: Zebrafish,X. tropicalis

Purification: Affinity Purified

Immunogen: between AA 1400 and 1450

Isotype: IgG

Application Details

Application Notes:

IP: 50 - 100 μL/mg lysate

WB: 1:10,000 - 1:25,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CNOT1

Target: CNOT1 (CCR4-NOT Transcription Complex, Subunit 1 (CNOT1))

Alternative Name: CNOT1

Background: Background: Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of emryonic stem (ES) cell identity. [taken from NCBI Entrez Gene (Gene ID: 23019)]. [taken from the Universal Protein Resource (UniProt) A5YKK6].

Gene ID: 23019

UniProt: A5YKK6

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Stem Cell Maintenance