METTL14 antibody (AA 25-75)

Catalog No. ABIN7452826

This Rabbit Polyclonal antibody specifically detects METTL14 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for METTL14 antibody (AA 25-75) (ABIN7452826)

Target: METTL14 (Methyltransferase Like 14 (METTL14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METTL14 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-METTL14 Antibody

Binding Specificity: AA 25-75

Purpose: Rabbit anti-METTL14 Antibody, Affinity Purified

Predicted Reactivity: Mouse,X. laevis,X. tropicalis,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 25 and 75

Isotype: IgG

Application Details

Application Notes:

IP: 50-100 μL/mg lysate

WB: 1:10,000 - 1:25,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for METTL14

Target: METTL14 (Methyltransferase Like 14 (METTL14))

Alternative Name: METTL14

Background: Background: METTL14 forms a heterodimer with METTL3, creating an N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity). [taken from the Universal Protein Resource (UniProt) Q9HCE5].

Gene ID: 57721

UniProt: Q9HCE5