Sestrin 2 antibody (AA 275-325)

Catalog No. ABIN7452840

This anti-Sestrin 2 antibody is a Rabbit Polyclonal antibody detecting Sestrin 2 in WB and IP. Suitable for Human.

Quick Overview for Sestrin 2 antibody (AA 275-325) (ABIN7452840)

Target: Sestrin 2 (SESN2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sestrin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Sestrin 2 Antibody

Binding Specificity: AA 275-325

Purpose: Rabbit anti-SESN2 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Orangutan

Purification: Affinity Purified

Immunogen: between AA 275 and 325

Isotype: IgG

Application Details

Application Notes:

IP: 50-100 μL/mg lysate

WB: 1:10,000 - 1:25,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Sestrin 2

Target: Sestrin 2 (SESN2)

Alternative Name: SESN2

Background: Background: SESN2 functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. SESN2 may negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1, and may positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1. May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress, and has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein.  [taken from the Universal Protein Resource (UniProt) P58004].

Gene ID: 83667

UniProt: P58004