BRD4 antibody (AA 1312-1362)

Catalog No. ABIN7453424

The Rabbit Polyclonal anti-BRD4 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect BRD4 in samples from Human and Mouse.

Quick Overview for BRD4 antibody (AA 1312-1362) (ABIN7453424)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-BRD4 Antibody

Binding Specificity: AA 1312-1362

Purpose: Rabbit anti-BRD4 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1312 and 1362

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4

Background: Background: Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.

Gene ID: 23476

NCBI Accession: NP_490597

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome