hPrp3p (AA 100-150) antibody

Catalog No. ABIN7453461

The Rabbit Polyclonal anti-hPrp3p antibody (ABIN7453461) specifically detects hPrp3p in IP, IHC (fp) and WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for hPrp3p (AA 100-150) antibody (ABIN7453461)

Target: hPrp3p

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)

Product Details

Binding Specificity: AA 100-150

Purpose: Rabbit anti-hPrp3p Antibody, Affinity Purified

Predicted Reactivity: Chicken,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 100 and 150

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7453461 has not performed satisfactorily when used for WB of hPrp3p in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of hPrp3p.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: hPrp3p

Background: Background: hPrp3p (human pre-mRNA splicing factor 3) was identified as a factor that associated with U4/U6 snRNPs which are components of the splicesome. hPrp3p is homologous to S. cerevisiae splicing factor Prp3p. Mutations in the PRPF3 gene are associated with retinitis pigmentosa-18. It has been determined that mutations in hPrp3p may alter the recognition motif for phosphorylation by casein kinase II (CKII) and that loss of phosphorylation may result in aberrant interactions within the U4/U6 snRNP complex.

Gene ID: 9129

NCBI Accession: NP_004689

UniProt: O43395