UBE2L3 antibody (C-Term)

Catalog No. ABIN7453475

The Rabbit Polyclonal anti-UBE2L3 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect UBE2L3 in samples from Human and Mouse.

Quick Overview for UBE2L3 antibody (C-Term) (ABIN7453475)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2L3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-UBE2L3 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-UBCH7 Antibody, Affinity Purified

Predicted Reactivity: Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 100 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate (efficiency in IP is low)

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for UBE2L3

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: UBCH7

Background: Background: Ubiquitin conjugating enzyme H7 (UBCH7) is an E2 enzyme of the three step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ubiquitination provides an important post-translational means to regulate protein abundance and activity. UBCH7 has been shown to be involved in the ubiquitination of p53.

Gene ID: 7332

NCBI Accession: NP_003338

UniProt: P68036