CDT1 antibody (C-Term)

Catalog No. ABIN7453476

This anti-CDT1 antibody is a Rabbit Polyclonal antibody detecting CDT1 in WB, IP and IHC (fp). Suitable for Human.

Quick Overview for CDT1 antibody (C-Term) (ABIN7453476)

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CDT1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-CDT1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 500 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:1,000 - 1:2,500

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CDT1

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Alternative Name: CDT1

Background: Background: CDT1 is involved in the formation of pre-replicative complexes (preRC) at origins of replication. Under tight regulation, CDT1 associates with chromatin during G1 in a dynamic manner to promote the loading of mini-chromosome maintenance (MCM) complex in preparation for replication. CDT1 is negatively regulated by ubiquitin-directed degradation and has been demonstrated to be protected from ubiquitination by the protein geminin.

Gene ID: 81620

NCBI Accession: NP_112190

UniProt: Q9H211

Pathways: MAPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA