eIF4AIII/EIF4A3 (AA 1-50) antibody

Catalog No. ABIN7453516

This Rabbit Polyclonal antibody specifically detects eIF4AIII/EIF4A3 in IHC (fp), IP and WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for eIF4AIII/EIF4A3 (AA 1-50) antibody (ABIN7453516)

Target: eIF4AIII/EIF4A3

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Binding Specificity: AA 1-50

Purpose: Rabbit anti-eIF4AIII/EIF4A3 Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine,Pig

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: eIF4AIII/EIF4A3

Background: Background: Eukaryotic initiation factor 4A-III is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. eIF4AIII is an ATP-dependent helicase and a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs.

Gene ID: 9775

NCBI Accession: NP_055555

UniProt: P38919