POLD1 antibody (AA 50-100)

Catalog No. ABIN7453541

The Rabbit Polyclonal anti-POLD1 antibody is suitable to detect POLD1 in samples from Human and Mouse. It has been validated for WB and IHC (fp).

Quick Overview for POLD1 antibody (AA 50-100) (ABIN7453541)

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-POLD1 Antibody

Binding Specificity: AA 50-100

Purpose: Rabbit anti-PolD1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 50 and 100

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for POLD1

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Alternative Name: PolD1

Background: Background: DNA polymerase delta catalytic subunit (PolD1) is the 125- kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair [taken from NCBI Entrez Gene (Gene ID: 5424)].

Gene ID: 5424

NCBI Accession: NP_002682

UniProt: P28340

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA