RPA1 antibody (AA 350-400)

Catalog No. ABIN7453556

The Rabbit Polyclonal anti-RPA1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect RPA1 in samples from Human and Mouse.

Quick Overview for RPA1 antibody (AA 350-400) (ABIN7453556)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-RPA1 Antibody

Binding Specificity: AA 350-400

Purpose: Rabbit anti-RPA70 Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 350 and 400

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA70

Background: Background: Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32 kDa (RPA2), and 14 kDa (RPA3). RPA1, the 70 kDa subunit, is an ssDNA binding protein that has been shown to be phosphorylated by ATM and ATR in response to DNA damage. In addition to a role in MMR, RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication.

Gene ID: 6117

NCBI Accession: NP_002936

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA