ARFGEF1 antibody (C-Term)

Catalog No. ABIN7453595

The Rabbit Polyclonal anti-ARFGEF1 antibody is suitable to detect ARFGEF1 in samples from Human and Mouse. It has been validated for WB, IP and IHC (fp).

Quick Overview for ARFGEF1 antibody (C-Term) (ABIN7453595)

Target: ARFGEF1 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1(brefeldin A-Inhibited) (ARFGEF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFGEF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-ARFGEF1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-BIG1/ARFGEF1 Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 1800 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:1,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for ARFGEF1

Target: ARFGEF1 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1(brefeldin A-Inhibited) (ARFGEF1))

Alternative Name: BIG1/ARFGEF1

Background: Background: BIG1/ARFGEF1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.

Gene ID: 10565

NCBI Accession: NP_006412

UniProt: Q9Y6D6

Pathways: Regulation of Actin Filament Polymerization, Regulation of Carbohydrate Metabolic Process