ARFGEF1 antibody (AA 1-200)
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- Target See all ARFGEF1 Antibodies
- ARFGEF1 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1(brefeldin A-Inhibited) (ARFGEF1))
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Binding Specificity
- AA 1-200
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ARFGEF1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Cross-Reactivity
- Mouse
- Predicted Reactivity
- Human,Rat,Dog,Cow,Sheep,Pig,Chicken
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human BIG1/ARFGEF1
- Isotype
- IgG
- Top Product
- Discover our top product ARFGEF1 Primary Antibody
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- ARFGEF1 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1(brefeldin A-Inhibited) (ARFGEF1))
- Alternative Name
- BIG1/ARFGEF1 (ARFGEF1 Products)
- Synonyms
- ARFGEF1 antibody, ARFGEP1 antibody, BIG1 antibody, P200 antibody, D130059B05Rik antibody, D730028O18Rik antibody, RGD1560793 antibody, ADP ribosylation factor guanine nucleotide exchange factor 1 antibody, brefeldin A-inhibited guanine nucleotide-exchange protein 1 antibody, ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) antibody, ARFGEF1 antibody, CpipJ_CPIJ004831 antibody, Arfgef1 antibody
- Background
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Synonyms: ADPribosylation factor guanine nucleotide exchange factor 1brefeldin A inhibited, ARFGEF1, ARFGEP1, Brefeldin A inhibited GEP 1, Brefeldin A inhibited guanine nucleotide exchange protein 1, p200 ARF GEP1, p200 ARF guanine nucleotide exchange factor, BIG1_HUMAN.
Background: Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways.
- Gene ID
- 10565
- Pathways
- Regulation of Actin Filament Polymerization, Regulation of Carbohydrate Metabolic Process
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