CPSF2 antibody (AA 450-500)

Catalog No. ABIN7453679

The Rabbit Polyclonal anti-CPSF2 antibody is suitable to detect CPSF2 in samples from Human and Mouse. It has been validated for WB, IP and IHC (fp).

Quick Overview for CPSF2 antibody (AA 450-500) (ABIN7453679)

Target: CPSF2 (Cleavage and Polyadenylation Specific Factor 2, 100kDa (CPSF2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CPSF2 Antibody

Binding Specificity: AA 450-500

Purpose: Rabbit anti-CPSF100 Antibody, Affinity Purified

Predicted Reactivity: Bovine

Purification: Affinity Purified

Immunogen: between AA 450 and 500

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CPSF2

Target: CPSF2 (Cleavage and Polyadenylation Specific Factor 2, 100kDa (CPSF2))

Alternative Name: CPSF100

Background: Background: CPSF100/CPSF2 (cleavage and polyadenylation specific factor 2, 100 kDa subunit) is a subunit of the cleavage and polyadenylation specificity factor (CPSF) involved in mammalian mRNA 3'-end processing. CPSF, along with CstF (cleavage and stimulation factor), is critical for the recognition of cis-acting sequence elements on the pre-mRNA that dictate the site of cleavage and subsequent polyadenylation. CPSF exists as a complex of four proteins that includes CPSF160/CPSF1, CPSF100/CPSF2, CPSF73/CPSF3, and CPSF30/CPSF4. CPSF100/CPSF2 and CPSF73/CPSF3 are both members of the metallo-beta-lactamase superfamily of zinc-dependent hydrolases.

Gene ID: 53981

NCBI Accession: NP_059133

UniProt: Q9P2I0