eIF4AIII/EIF4A3 (AA 125-175) antibody

Catalog No. ABIN7453697

The Rabbit Polyclonal anti-eIF4AIII/EIF4A3 antibody is suitable to detect eIF4AIII/EIF4A3 in samples from Human and Mouse. It has been validated for IHC (fp), IP and WB.

Quick Overview for eIF4AIII/EIF4A3 (AA 125-175) antibody (ABIN7453697)

Target: eIF4AIII/EIF4A3

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Binding Specificity: AA 125-175

Purpose: Rabbit anti-eIF4AIII/EIF4A3 Antibody, Affinity Purified

Predicted Reactivity: Rat,Zebrafish,X. laevis,X. tropicalis,Chicken,Bovine,Pig

Purification: Affinity Purified

Immunogen: between AA 125 and 175

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: eIF4AIII/EIF4A3

Background: Background: Eukaryotic initiation factor 4A-III is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. eIF4AIII is an ATP-dependent helicase and a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs.

Gene ID: 9775

NCBI Accession: NP_055555

UniProt: P38919