NEDD4-2 antibody (AA 275-325)

Catalog No. ABIN7453739

The Rabbit Polyclonal anti-NEDD4-2 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect NEDD4-2 in samples from Human and Mouse.

Quick Overview for NEDD4-2 antibody (AA 275-325) (ABIN7453739)

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD4-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-NEDD4-2 Antibody

Binding Specificity: AA 275-325

Purpose: Rabbit anti-NEDD4L Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 275 and 325

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for NEDD4-2

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Alternative Name: NEDD4L

Background: Background: NEDD4L (neural precursor cell expressed, developmentally down-regulated 4-like) functions as an E3 ubiquitin-protein ligase. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. It also promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5 [taken from the Universal Protein Resource (UniProt) wwwuniprotorg/uniprot/Q96PU5].

Gene ID: 23327

NCBI Accession: NP_001138439

UniProt: Q96PU5

Pathways: Negative Regulation of Transporter Activity