Nucleolin antibody (AA 525-575)

Catalog No. ABIN7453747

This Rabbit Polyclonal antibody specifically detects Nucleolin in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Nucleolin antibody (AA 525-575) (ABIN7453747)

Target: Nucleolin (NCL)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Nucleolin Antibody

Binding Specificity: AA 525-575

Purpose: Rabbit anti-Nucleolin (NCL) Antibody, Affinity Purified

Predicted Reactivity: Rat,Golden hamster,Orangutan

Purification: Affinity Purified

Immunogen: between AA 525 and 575

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin/NCL

Background: Background: Nucleolin (NCL) is a major nucleolar phosphoprotein that is involved in pre-RNA transcription and the synthesis and maturation of ribosomes. NCL can bind to histone H1 to induce chromatin condensation and may play an additional role in transcriptional elongation.

Gene ID: 4691

NCBI Accession: NP_005372

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization