Prohibitin antibody (AA 222-272)

Catalog No. ABIN7453838

The Rabbit Polyclonal anti-Prohibitin antibody has been validated for WB, IHC (fp) and IP. It is suitable to detect Prohibitin in samples from Human.

Quick Overview for Prohibitin antibody (AA 222-272) (ABIN7453838)

Target: Prohibitin (PHB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Product Details anti-Prohibitin Antibody

Binding Specificity: AA 222-272

Purpose: Rabbit anti-Prohibitin Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 222 and 272

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: Prohibitin

Background: Background: Prohibitin (PHB) has been identified as a tumor suppressor protein that can repress E2F-mediated transcription and arrest cell cycle progression. It has recently been shown to associate with members of the minichromosome maintenance complex such as MCM2, MCM5, and MCM7 and therefore is thought to impart its growth inhibitory effects via association with the replication machinery. Prohibitin is also implicated in regulating mitochondrial respiration and playing a role in aging.

Gene ID: 5245

NCBI Accession: NP_002625

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling