BUB1B antibody (AA 1-50)

Catalog No. ABIN7453860

The Rabbit Polyclonal anti-BUB1B antibody (ABIN7453860) specifically detects BUB1B in WB, IP and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for BUB1B antibody (AA 1-50) (ABIN7453860)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-BUB1B Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-BubR1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BubR1

Background: Background: BubR1 is cell cycle checkpoint kinase and component of the spindle checkpoint machinery. BubR1 and other spindle checkpoint proteins ensure that segregation does not proceed until all replicated chromatids are associated with a functional bipolar spindle. BubR1 is part of the mitotic checkpoint complex that also includes Bub3, Mad2, and Cdc20. In this trimer BubR1 functions to inhibit the anaphase promoting complex (APC) that promotes chromosome segregation. BubR1 is suggested to be a paralog of Bub1.

Gene ID: 701

NCBI Accession: NP_001202

UniProt: O60566