HSP27 antibody (AA 150-200)

Catalog No. ABIN7453910

The Rabbit Polyclonal anti-HSP27 antibody (ABIN7453910) specifically detects HSP27 in WB, IP and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for HSP27 antibody (AA 150-200) (ABIN7453910)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-HSP27 Antibody

Binding Specificity: AA 150-200

Purpose: Rabbit anti-HSP27 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 150 and 200

Isotype: IgG

Application Details

Application Notes:

IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Background: Background: Heat shock protein 27 (HSP27) is involved in stress resistance and actin organization [taken from the Universal Protein Resource (UniProt) P04792].

Gene ID: 3315

NCBI Accession: NP_001531

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling