RAN antibody (AA 175-225)

Catalog No. ABIN7453926

This Rabbit Polyclonal antibody specifically detects RAN in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for RAN antibody (AA 175-225) (ABIN7453926)

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAN antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-RAN Antibody

Binding Specificity: AA 175-225

Purpose: Rabbit anti-RAN Antibody, Affinity Purified

Predicted Reactivity: Rat,X. laevis,Chicken,Bovine,Dog,Orangutan

Purification: Affinity Purified

Immunogen: Between AA 175 and 225

Isotype: IgG

Application Details

Application Notes:

IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RAN

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Alternative Name: RAN

Background: Background: RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor [taken from NCBI Entrez Gene (Gene ID: 5901)].

Gene ID: 5901

NCBI Accession: NP_006316

UniProt: P62826

Pathways: Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus