REST antibody (AA 800-850)

Catalog No. ABIN7454366

This anti-REST antibody is a Rabbit Polyclonal antibody detecting REST in IP and IHC (fp). Suitable for Human.

Quick Overview for REST antibody (AA 800-850) (ABIN7454366)

Target: REST (RE1-Silencing Transcription Factor (REST))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-REST Antibody

Binding Specificity: AA 800-850

Purpose: Rabbit anti-REST Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 800 and 850

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. Use rabbit anti-REST antibody A300-540A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: REST

Background: Background: RE1-Silencing Transcription Factor (REST) is a transcriptional repressor that silences neuronal gene expression in non-neuronal cells by binding to the neuron-restrictive silencer element (NRSE). REST is a Kruppel-type zinc finger transcription factor that mediates repression through its interaction with the mSin3 and CoREST corepressors and the HDAC1 and HDAC2 histone deacetylases.

Gene ID: 5978

NCBI Accession: NP_005603

UniProt: Q13127

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity