EIF2A antibody (AA 450-500)

Catalog No. ABIN7454423

This anti-EIF2A antibody is a Rabbit Polyclonal antibody detecting EIF2A in IP and IHC (fp). Suitable for Human.

Quick Overview for EIF2A antibody (AA 450-500) (ABIN7454423)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-EIF2A Antibody

Binding Specificity: AA 450-500

Purpose: Rabbit anti-eIF2A Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 450 and 500

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-eIF2A antibody A301-949A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: eIF2A

Background: Background: Eukaryotic initiation factor 2A (eIF2A) directs binding of the intiator methionyl-tRNA [Met-tRNA(i)] to 40 S ribosomal subunits in a codon dependent manner. It is a 65 kDa protein that was originally believed to be the functional homolog of prokaryotic IF2. eIF2A displays functional qualities similar to eIF2 which exists as complex of 3 subunits (eIF2-alpha, beta, and gamma), however comparison of eIF2A and eIF2 has shown that these two intitiation factors also display distinct functional abilities.

Gene ID: 83939

NCBI Accession: NP_114414

UniProt: Q9BY44

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly