BTBD12 antibody (AA 1784-1834)

Catalog No. ABIN7454441

The Rabbit Polyclonal anti-BTBD12 antibody (ABIN7454441) specifically detects BTBD12 in IP and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for BTBD12 antibody (AA 1784-1834) (ABIN7454441)

Target: BTBD12 (BTB (POZ) Domain Containing 12 (BTBD12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTBD12 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-BTBD12 Antibody

Binding Specificity: AA 1784-1834

Purpose: Rabbit anti-BTBD12 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1784 and 1834

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 10 μg/mg lysate

WB: Not recommended. A302-270A has not performed satisfactorily when used for WB of BTBD12 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BTBD12.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BTBD12

Target: BTBD12 (BTB (POZ) Domain Containing 12 (BTBD12))

Alternative Name: BTBD12

Background: Background: BTBD12 (BTBD12 domain-containing protein 12) has been identified as the human ortholog of the yeast Slx4p and drosophila MUS312 DNA repair factors. BTBD12 is an ATM/ATR checkpoint substrate that functions as part of a Holliday junction resolvase and is important for DNA interstrand crosslink repair.

Gene ID: 84464

NCBI Accession: NP_115820

UniProt: Q8IY92

Pathways: DNA Damage Repair