RAD23B antibody (AA 125-175)

Catalog No. ABIN7454444

This anti-RAD23B antibody is a Rabbit Polyclonal antibody detecting RAD23B in IP and IHC (fp). Suitable for Human.

Quick Overview for RAD23B antibody (AA 125-175) (ABIN7454444)

Target: RAD23B (RAD23 Homolog B (RAD23B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD23B antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-RAD23B Antibody

Binding Specificity: AA 125-175

Purpose: Rabbit anti-RAD23B Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 125 and 175

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 10 μg/mg lysate

WB: Not recommended. Use rabbit anti-RAD23B antibody A302-306A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RAD23B

Target: RAD23B (RAD23 Homolog B (RAD23B))

Alternative Name: RAD23B

Background: Background: RAD23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. RAD23B was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Gene ID: 5887

NCBI Accession: NP_002865

UniProt: P54727

Pathways: DNA Damage Repair