HSF1 antibody (AA 425-475)

Catalog No. ABIN7454482

The Rabbit Polyclonal anti-HSF1 antibody has been validated for IP and IHC (fp). It is suitable to detect HSF1 in samples from Human.

Quick Overview for HSF1 antibody (AA 425-475) (ABIN7454482)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-HSF1 Antibody

Binding Specificity: AA 425-475

Purpose: Rabbit anti-HSF1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 425 and 475

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background: Background: Heat shock factor protein 1 (HSF1) is a DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked [taken from the Universal Protein Resource (UniProt) Q00613].

Gene ID: 3297

NCBI Accession: NP_005517

UniProt: Q00613