DHX9 antibody (AA 200-250)

Catalog No. ABIN7454523

This Rabbit Polyclonal antibody specifically detects DHX9 in WB and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for DHX9 antibody (AA 200-250) (ABIN7454523)

Target: DHX9 (ATP-Dependent RNA Helicase A (DHX9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-DHX9 Antibody

Binding Specificity: AA 200-250

Purpose: Rabbit anti-DHX9 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 200 and 250

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended. Use rabbit anti-DHX9 antibody A300-855A.

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DHX9

Target: DHX9 (ATP-Dependent RNA Helicase A (DHX9))

Alternative Name: DHX9

Background: Background: DHX9 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAH box proteins also exhibit functions involved in transcriptional regulation. DHX9 has been shown to act as a bridge between CREB-binding protein (CBP) and RNA polymerase II to coactivate CBP-dependent transcription. A similar observation has been reported for an interaction with BRCA1. DHX9 also exhibits activities associated with nuclear export of retroviral RNAs, translation, RNA editing, and snRNP assembly.

Gene ID: 1660

UniProt: Q08211