GAPDH antibody (AA 175-225)

Catalog No. ABIN7454568

This Goat Polyclonal antibody specifically detects GAPDH in WB and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for GAPDH antibody (AA 175-225) (ABIN7454568)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-GAPDH Antibody

Binding Specificity: AA 175-225

Purpose: Goat anti-GAPDH Antibody, Affinity Purified

Predicted Reactivity: Rat,D. melanogaster,Zebrafish,X. laevis,Chicken,Sheep,Bovine,Rabbit,Guinea pig_10141,Pig,Golden hamster,Cat,Orangutan

Purification: Affinity Purified

Immunogen: Between AA 175 and 225

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended

WB: 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Background: In the process of carbohydrate metabolism, GAPDH (glyceraldehydes-3-phosphate dehydrogenase) catalyzes the oxidative phosphorylation of glyceraldehydes-3-phosphate to 1,3-diphosphoglycerate in the presence of nicotinamide adenine dinucleotide (NAD) and inorganic phosphate. In addition to its role in the oxidation of glucose, GAPDH has been shown to be involved in other cellular processes such as vesicle transport, DNA repair, DNA replication, cytoskeletal modulation, and tRNA export.

Gene ID: 2597

NCBI Accession: NP_002037

UniProt: P04406