DNMT3A antibody (AA 125-175)

Catalog No. ABIN7454575

The Rabbit Polyclonal anti-DNMT3A antibody has been validated for WB and IHC (fp). It is suitable to detect DNMT3A in samples from Human and Mouse.

Quick Overview for DNMT3A antibody (AA 125-175) (ABIN7454575)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-DNMT3A Antibody

Binding Specificity: AA 125-175

Purpose: Rabbit anti-DNMT3A Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 125 and 175

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNMT3A

Background: Background: DNA cytosine methyltransferase 3A2 (DNMT3A) is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. DNMT3A modifies DNA in a non-processive manner and also methylates non-CpG sites. It may preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. DNMT3A plays a role in paternal and maternal imprinting and is required for methylation of most imprinted loci in germ cells [taken from the Universal Protein Resource (UniProt) Q9Y6K1].

Gene ID: 1788

NCBI Accession: NP_783328

UniProt: Q9Y6K1