Menin antibody (C-Term)

Catalog No. ABIN7454589

This Rabbit Polyclonal antibody specifically detects Menin in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for Menin antibody (C-Term) (ABIN7454589)

Target: Menin (MEN1) (Multiple Endocrine Neoplasia 1 (MEN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Menin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Menin Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-Menin Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine,Dog

Purification: Affinity Purified

Immunogen: between AA 575 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:10,000 - 1:25,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Menin

Target: Menin (MEN1) (Multiple Endocrine Neoplasia 1 (MEN1))

Alternative Name: Menin

Background: Background: Menin is the gene product of the putative tumor suppressor MEN1 associated with the syndrome termed multiple endocrine neoplasia type 1 that may function in the regulation of DNA synthesis or the response to DNA damage. Menin has been shown to interact with JunD, a member of the AP-1 heterodimer and inhibit transcription. Menin has also been shown to interact with Pem, FANCD2, RPA, NMMHCII-A, GFAP, vimentin, Hsp70, NfkappaB proteins, Smads, mSin3A and is part of the MLL2 and MLL1 Set1-like histone H3 K4 methyltransferase complex where it interacts directly with ASH2, Rbbp5, and WDR5. As part of the MLL complexes, menin may mediate its tumor suppressor activity via the regulation of histone methylation of HOX and CDK inhibitor genes. Alternative names for Menin are MEAI, multiple endocrine adenomatosis 1, suppressor Candidate Gene 2, and SCG2.

Gene ID: 4221

NCBI Accession: NP_000235

UniProt: O00255

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity