MCM5 antibody (AA 1-50)

Catalog No. ABIN7454594

The Rabbit Polyclonal anti-MCM5 antibody (ABIN7454594) specifically detects MCM5 in WB, IP and IHC (fp). The antibody is reactive with Human and Mouse samples.

Quick Overview for MCM5 antibody (AA 1-50) (ABIN7454594)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MCM5 Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-MCM5 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 4 μg/mg lysate. This antibody is efficient at pulling down the MCM2-7 complex.

WB: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5

Background: Background: The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM5 can form a subcomplex with MCM3. MCM5 also appears to play a role in transcription. MCM5 has been shown to interact with the signal transducer and activator of transcription 1 (STAT1) and demonstrated to be essential for STAT1 target gene transcription.

Gene ID: 4174

NCBI Accession: NP_006730

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA