DDB1 antibody (C-Term)

Catalog No. ABIN7454634

This Rabbit Polyclonal antibody specifically detects DDB1 in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for DDB1 antibody (C-Term) (ABIN7454634)

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-DDB1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-DDB1 Antibody, Affinity Purified

Predicted Reactivity: Rat,X. laevis,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 1100 and C-term

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Alternative Name: DDB1

Background: Background: DDB1 (DNA Damage Binding Protein 1) is a subunit of the DNA damage-binding protein complex (DDB) involved in DNA repair. As part of a complex with Cul4-based ubiquitin ligase, Cul4-Ddb1 can directly modify chromatin through the ubiquitination of histones as well as ubiquitinate other chromatin bound factors. A deficiency in Cul4-Ddb1 ubiquitin ligase has been associated with xeroderma pigmentosum, a heritable disease characterized by defects in nucleotide excision repair and skin cancer development.

Gene ID: 1642

UniProt: Q16531, Q13289

Pathways: DNA Damage Repair