AF9 antibody (AA 250-300)

Catalog No. ABIN7454650

The Rabbit Polyclonal anti-AF9 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect AF9 in samples from Human.

Quick Overview for AF9 antibody (AA 250-300) (ABIN7454650)

Target: AF9 (MLLT3) (Protein AF-9 (MLLT3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AF9 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-AF9 Antibody

Binding Specificity: AA 250-300

Purpose: Rabbit anti-AF9 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 250 and 300

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 1 - 4 μg/mg lysate

WB: 1:5,000 - 1:15,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for AF9

Target: AF9 (MLLT3) (Protein AF-9 (MLLT3))

Alternative Name: AF9

Background: Background: The human AF9 gene is one of the most common fusion partner genes with the MLL (mixed lineage leukemia) gene resulting in AF9/MLL translocations and leukemogenesis. AF9 is highly homologous to the gene product of ENL, another common MLL fusion partner gene. The function of AF9 is not known but through studies of ENL it is proposed to be a nuclear transactivating factor.

Gene ID: 4300

NCBI Accession: NP_004520

UniProt: P42568