CHD8 antibody (AA 2252-2302)

Catalog No. ABIN7454728

The Rabbit Polyclonal anti-CHD8 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect CHD8 in samples from Human and Mouse.

Quick Overview for CHD8 antibody (AA 2252-2302) (ABIN7454728)

Target: CHD8 (Chromodomain Helicase DNA Binding Protein 8 (CHD8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CHD8 Antibody

Binding Specificity: AA 2252-2302

Purpose: Rabbit anti-CHD8 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 2252 and 2302

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 to 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CHD8

Target: CHD8 (Chromodomain Helicase DNA Binding Protein 8 (CHD8))

Alternative Name: CHD8

Background: Background: CHD8 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD8 has been reported to interact with duplin, a protein involved in the wnt signaling pathway, and CTCF, a protein involved in heterochromatin formation.

Gene ID: 57680

NCBI Accession: NP_065971

UniProt: Q9HCK8

Pathways: Chromatin Binding