RRAGA antibody (AA 1-50)

Catalog No. ABIN7454752

The Rabbit Polyclonal anti-RRAGA antibody has been validated for WB, IP and IHC (fp). It is suitable to detect RRAGA in samples from Human.

Quick Overview for RRAGA antibody (AA 1-50) (ABIN7454752)

Target: RRAGA (Ras-Related GTP Binding A (RRAGA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRAGA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-RRAGA Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-FIP1 Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RRAGA

Target: RRAGA (Ras-Related GTP Binding A (RRAGA))

Alternative Name: FIP1

Background: Background: FIP1 was originally identified as Rhe (rearranged in hypereosinophilia) and part of a fusion protein encoded by a genetic rearrangement between the N-terminal region of FIP1 (Rhe) and the C-terminal region of the platelet-derived growth factor receptor alpha (PDGFRalpha). The fusion protein exhibits aberrant tyrosine kinase activity and is associated with hypereosinophilic syndrome (HES), a rare hematologic disorder characterized by an overproduction of eosinophils that results in organ damage. FIP1 was also identified as FIP1-like 1 in a database search for a human homolog of the yeast Fip1p (factor interacting with PAP), a subunit of the yeast 3'-end processing complex, CPF (cleavage and polyadenylation factor). Human FIP1 was similarly found to interact with PAP and CPSF160 as part of the human CPSF (cleavage and polyadenylation specificity factor) complex. In association with CPSF160, FIP1 is able to recognize U-rich elements on pre-mRNA and stimulate PAP activity.

Gene ID: 81608

NCBI Accession: NP_112179

UniProt: Q6UN15