SF3A1 antibody (AA 350-400)

Catalog No. ABIN7454763

This Rabbit Polyclonal antibody specifically detects SF3A1 in WB, IP and IHC (fp). It exhibits reactivity toward Human and Mouse.

Quick Overview for SF3A1 antibody (AA 350-400) (ABIN7454763)

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-SF3A1 Antibody

Binding Specificity: AA 350-400

Purpose: Rabbit anti-SF3a120/SAP114 Antibody, Affinity Purified

Predicted Reactivity: Bovine

Purification: Affinity Purified

Immunogen: between AA 350 and 400

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SF3A1

Target: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Alternative Name: SF3a120/SAP114

Background: Background: SF3a120/SAP114 is a 120 kDa subunit of the heterotrimeric splicing factor 3a (SF3A) protein complex. SF3A along with SF3B and a 12S RNA subunit are components of the 17S U2 small nuclear ribonucleoprotein complex (U2 snRNP) involved in pre-mRNA splicing. SF3a120/SAP114 bears two SURP motif repeats and one ubiquitin-like domain. SURP motifs are found among proteins involved in regulated mRNA splicing. The SURP motifs along with the ubiquitin-like domain of SF3a120/SAP114 appear to function in the heterotrimeric formation between the 120, 60 and 66 kDa subunits.

Gene ID: 10291

NCBI Accession: NP_005868

UniProt: Q15459

Pathways: Ribonucleoprotein Complex Subunit Organization