EEF2K antibody (AA 1-50)

Catalog No. ABIN7454772

This Rabbit Polyclonal antibody specifically detects EEF2K in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for EEF2K antibody (AA 1-50) (ABIN7454772)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-EEF2K Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-eEF2 Kinase Antibody, Affinity Purified

Predicted Reactivity: Rat

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: eEF2 Kinase

Background: Background: Eukaryotic elongation factor-2 kinase (eEF2 kinase) is a calcium- and calmodulin-dependent kinase that phosphorylates eukaryotic elongation factor 2 (eEF2), a non-ribosomal factor that mediates ribosomal translocation. eEF2 is regulated by MAP kinase signaling and the mTOR signaling pathway. eEF2 is phosphorylated in response to stimuli that increases energy demand or reduces its supply. Phosphorylation inhibits eEF2 activity to conserve energy.

Gene ID: 29904

NCBI Accession: NP_037434

UniProt: O00418

Pathways: AMPK Signaling