Notch1 antibody (AA 2505-2555)

Catalog No. ABIN7454790

This Rabbit Polyclonal antibody specifically detects Notch1 in WB, IP and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Notch1 antibody (AA 2505-2555) (ABIN7454790)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Notch1 Antibody

Binding Specificity: AA 2505-2555

Purpose: Rabbit anti-Notch1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 2505 and 2555

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: Notch1

Background: Background: Notch1 is the human homolog of the D. melanogaster Notch gene first reported as the gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch protein is a transmembrane receptor that mediates juxtacrine signals between adjacent cells. Mammalian Notch1 ligands include Delta-like and Jagged. Upon ligand binding, Notch1 undergoes two proteolytic cleavage events that result in the release of the intracellular Notch1 domain and its translocation to the nucleus where it can participate in gene transactivation. Notch 1 signals mediate cellular fate, differentiation, and development.

Gene ID: 4851

NCBI Accession: NP_060087

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development