EML4 antibody (AA 931-981)

Catalog No. ABIN7454794

The Rabbit Polyclonal anti-EML4 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect EML4 in samples from Human.

Quick Overview for EML4 antibody (AA 931-981) (ABIN7454794)

Target: EML4 (Echinoderm Microtubule Associated Protein Like 4 (EML4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EML4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-EML4 Antibody

Binding Specificity: AA 931-981

Purpose: Rabbit anti-EML4 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 931 and 981

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for EML4

Target: EML4 (Echinoderm Microtubule Associated Protein Like 4 (EML4))

Alternative Name: EML4

Background: Background: Echinoderm microtubule associated protein like 4 (EML4) has been identified as part of a gene fusion with the kinase domain of the anaplastic lymphoma receptor tyrosine kinase (ALK) in non-small cell lung cancer. EML4 associates with in vitro polymerized microtubules. In the cell, phosphorylated EML4 associates with the mitotic spindle and is essential for proliferation and microtubule network formation.

Gene ID: 27436

NCBI Accession: NP_061936

UniProt: Q9HC35